{"product_id":"9781071606186","title":"Springer Protocols Handbooks","description":"\u003ch1\u003eSpringer Protocols Handbooks\u003c\/h1\u003e \u003ch2\u003eIslam, M. Tofazzal; Bhowmik, Pankaj K.; Molla, Kutubuddin A.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cdiv\u003eThis volume details the fundamentals of the \u003ci\u003eCRISPR-Cas system\u003c\/i\u003e, and its protocols illustrate advances in \u003ci\u003eCRISPR-Cas\u003c\/i\u003e techniques for efficient genome editing. Introductory chapters provide a wide horizon of \u003ci\u003eCRISPR\/Cas-based methods\u003c\/i\u003e and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. \u003c\/div\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and cutting-edge, \u003ci\u003eCRISPR-Cas Methods \u003c\/i\u003eaims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2020-07-05\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071606186\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-0616-2\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 278\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44806865223820,"sku":"9781071606186","price":116.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071606186.jpg?v=1775764395","url":"https:\/\/lateknightbooks.com\/products\/9781071606186","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}