This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.