This detailed new edition combines a wide selection of experimental methods for plant cold acclimation, deacclimation, and cold memory, ranging from the whole plant level of ecology and breeding to molecular profiling and the detailed analysis of specific proteins, with many levels of investigated complexity in between. The collection updates methodology from the first edition while adding chapters on numerous key advances in the field. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.