{"product_id":"9781071607572","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eNiopek, Dominik\u003c\/h2\u003e \u003cp\u003eThis volume provides cutting edge protocols for using optogenetic and opto-chemical techniques across a wide spectrum of applications. Chapters guide readers through methods in neuro-optogenetics, including methods to dissect local and long-range connectivity ex vivo, investigate G-protein coupled receptors, study astrocyte-neuron networks in vitro and in vivo, employ upconversion nanoparticles for transcranial optogenetic stimulation, and use Adeno-associated virus (AAV) for delivery of optogenetic constructs. At the same time, this book covers a range of methods for non-neuronal optogenetics, including various applications of light-oxygen-voltage domains or cryptochromes for control of nucleocytoplasmic transport, transgene expression and chromatin recruitment. The book further presents protocols for traceless light-control of gene expression with melanopsin, light-inducible cAMP production, use of light-regulated receptor tyrosine kinases (Opto-RTKs), synthesis of light-dependent biomaterials, and highlights emerging tools at the intersection of optogenetics and CRISPR. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eAuthoritative and cutting-edge, \u003ci\u003ePhotoswitching Proteins: Methods and Protocols \u003c\/i\u003eaims to provide a comprehensive list of methods and tools to investigate highly dynamic pathways and behaviors in cells and animals using optogenetics.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2021-07-11\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071607572\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-0755-8\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 287\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":46543186919564,"sku":"9781071607572","price":107.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071607572.jpg?v=1772848682","url":"https:\/\/lateknightbooks.com\/products\/9781071607572","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}