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This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis.
Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism.
Published by: Humana
Publication Date: 2021-07-31
Format: Hardcover
ISBN-13: 9781071616567
DOI: 10.1007/978-1-0716-1657-4
Dimensions: 254cm x178cm
Pages: 397