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Springer Protocols Handbooks

Springer Protocols Handbooks: Volume 2

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Springer Protocols Handbooks: Volume 2

Islam, M. Tofazzal; Molla, Kutubuddin Ali

This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. 

 Authoritative and cutting-edge,  CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a  holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism.


The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Details

Published by: Humana

Publication Date: 2021-07-31

Format: Hardcover

ISBN-13: 9781071616567

DOI: 10.1007/978-1-0716-1657-4

Dimensions: 254cm x178cm

Pages: 397

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