Methods in Molecular Biology: Methods and Protocols

Ng, Charlotte; Piscuoglio, Salvatore

This volume provides practical guidance on a variety of techniques and steps to ensure successful variant calling. Chapters detail methods for variant calling from single-nucleotide variants to structural variants, variant calling in specialized data types such as RNA-seq and UMI-tagged sequencing, alignment-free genotyping and SNP calling, variant detection in single-cell DNA sequencing data, variant annotation, and preanalytical quality control to ensure successful variant calling. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists step-by-step protocol to execute the algorithms, describes the input and output data, and includes tips on troubleshooting and known pitfalls.

Details

Published by: Humana

Publication Date: 2022-06-26

Format: Paperback

ISBN-13: 9781071622957

DOI: 10.1007/978-1-0716-2293-3

Dimensions: 254cm x178cm

Pages: 354