Methods in Molecular Biology: Methods and Protocols

Santamaría, Enrique; Fernández-Irigoyen, Joaquín

This fully updated book explores cerebrospinal fluid (CSF) molecular profiles in normal and pathological states, with a focus on mass spectrometry and different protein methods currently used in neurobiological disciplines. The book includes CSF sample preparation methods, shotgun and targeted proteomic workflows, as well as methods to characterize specific posttranslational modifications to achieve finer molecular resolution in proteomic studies of CSF. Additional chapters delve into experimental -omics strategies applied to the CSF lipidome and metabolome, as well as bioinformatic pipelines useful for analyzing and integrating the molecular information derived from high-throughput -omics experiments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols, Second Edition serves as an ideal resource for researchers beginning their journey into CSF molecular profiles, as well as for established researchers seeking valuable insight into the growing utility of proteomics and metabolomics in neuroscience.

Details

Published by: Humana

Publication Date: 2025-04-02

Format: Hardcover

ISBN-13: 9781071644614

DOI: 10.1007/978-1-0716-4462-1

Dimensions: 254cm x178cm

Pages: 334