{"product_id":"9781071647783","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eGarcía-Ortega, Xavier; Glieder, Anton; Kovar, Karin; Rieder, Lukas\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003eThis volume provides readers with a useful and comprehensive resource of laboratory protocols for working with \u003cem\u003ePichia pastoris\u003c\/em\u003e. The chapters in this book are organized into seven parts: i\u003cspan lang=\"DE-AT\" style=\"mso-ansi-language: DE-AT;\"\u003entroduction, bioinformatics, c\u003c\/span\u003eell and systems biology, gene expression cassettes and vectors, \u003cem\u003ePichia pastoris\u003c\/em\u003e expression strains, u\u003cspan lang=\"DE-AT\" style=\"mso-ansi-language: DE-AT;\"\u003epstream bioprocessing, and d\u003c\/span\u003eownstream processing and product analysis. This book covers basic and newer laboratory methods, starting from the gene and genetic construct to mature (yet reasonable) amounts of a recombinant product being produced, purified, and characterized. Written in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cbr\u003eCutting-edge and thorough, \u003cem\u003ePichia pastoris: Methods and Protocols \u003c\/em\u003eis a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2025-10-02\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071647783\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-4779-0\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 682\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44398661009548,"sku":"9781071647783","price":161.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071647783.jpg?v=1772892435","url":"https:\/\/lateknightbooks.com\/products\/9781071647783","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}