{"product_id":"9781071648230","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eMead, Jan R.; Arrowood, Michael J.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\" style=\"mso-ansi-language: EN-CA;\"\u003eThis second edition volume expands on the previous edition with new chapters discussing the latest \u003c\/span\u003eprotocols that encompass the detection, characterization, and genomic analysis of \u003cem\u003eCryptosporidium. \u003c\/em\u003eThe first few chapters in this book focus on diagnostic assays, such as traditional microscopy-based techniques, nucleic acid-based molecular detection, and immunoassay-based methodologies. Several chapters cover whole genome sequencing methodologies, genotyping approaches, and novel techniques such as hybridization bait capture analyses. A significant portion of this book is dedicated to functional genomics and experimental models, with discussions on tools used for genetic manipulation (including CRISPR\/cas9), gene silencing, and host-pathogen interactions. The volume concludes with chapters exploring drug discovery and testing, highlighting high-content screening methods, phenotypic assays, and efficacy testing of potential disinfectants and therapeutics. Written in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cbr\u003eCutting-edge and comprehensive, \u003cem\u003eCryptosporidium: Methods and Protocols, Second Edition \u003c\/em\u003eis a valuable tool for researchers interested in gaining more insight on foundational and innovative \u003cem\u003eCryptosporidium\u003c\/em\u003e research, which will inspire future advancements in this field.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2025-10-28\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781071648230\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-0716-4824-7\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 405\u003c\/p\u003e ","brand":"Springer US","offers":[{"title":"Default Title","offer_id":44309254406284,"sku":"9781071648230","price":179.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071648230.jpg?v=1772892447","url":"https:\/\/lateknightbooks.com\/products\/9781071648230","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}