This volume explores protocols and perspectives for the design, production, characterization, functional evaluation, and clinical translation of bispecific antibodies (BsAbs). Beginning with a variety of platforms for generating BsAbs, the book continues with sections covering protocols to evaluate T-cell-dependent bispecific antibodies, modeling and simulation approaches to support clinical development of T-cell-engaging BsAbs, as well as clinical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bispecific Antibodies: Methods and Protocols aims to bridge the gap between molecular design and translational success by equipping investigators with the technical know-how and contextual understanding needed to show each method’s relevance to therapeutic development.