This fourth edition volume expands on the previous editions by providing readers with a comprehensive guide to the latest foundational and advanced techniques used by researchers in the field. This book covers the construction of diverse antibody libraries including naïve, synthetic, and immune types and candidate selection using phage, yeast, or mammalian display systems. This book also discusses in silico approaches such as computational design, epitope prediction, and deep learning to improve antibody discovery and characterization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Antibody Engineering: Methods and Protocols, Fourth Edition is a valuable resource that provides scientists with an extensive toolbox to generate the next generation of antibody-based molecules for research, diagnostics, and therapeutic applications.

Chapter 1 is available Open Access under a Creative Commons Attribution 4.0. International License via link.springer.com