{"product_id":"9781071653272","title":"Clostridioides difficile Methods and Protocols","description":"\u003ch3\u003eMethods in Molecular Biology\u003c\/h3\u003e\u003ch1\u003eClostridioides difficile\u003c\/h1\u003e\u003ch2\u003eMethods and Protocols\u003c\/h2\u003e\u003ch3\u003eKurt H. Piepenbrink | Jennifer M. Auchtung\u003c\/h3\u003e\u003cdiv\u003e\u003cb\u003eScience \/ Life Sciences \/ Microbiology\u003c\/b\u003e\u003c\/div\u003e\u003cbr\u003e\u003cdiv\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\" style=\"mso-ansi-language: EN-CA;\"\u003eThis volume discusses the latest advancements on \u003c\/span\u003eapproaches used to characterize \u003cem\u003eC. difficile\u003c\/em\u003e physiology, including new methods for genetic modifications and novel models for studying host-pathogen interactions. The chapters in this book cover various topics such as FASTQ\u003cspan lang=\"EN-CA\" style=\"mso-ansi-language: EN-CA;\"\u003e; CRISPR-AsCas12a and dAsCas 12a-mediated gene knockout and knockdown in \u003cem\u003eClostridioides difficile; \u003c\/em\u003epreparation of spores with 70:30 method; measuring \u003cem\u003eC. difficile\u003c\/em\u003e adhesion to mucosal surfaces; and isolating \u003cem\u003eC. difficile \u003c\/em\u003efrom animal samples. \u003c\/span\u003eWritten in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cspan lang=\"EN-CA\" style=\"mso-ansi-language: EN-CA;\"\u003e\u003cbr\u003e\u003c\/span\u003eCutting-edge and practical, \u003cem\u003eClostridioides difficile: Methods and Protocols \u003c\/em\u003eis a valuable resource for researchers interested in learning more about this field. Gaining a deeper understanding of \u003cem\u003eC. difficile \u003c\/em\u003ephysiology is crucial for developing new therapies for treatment and predicting the future evolution of this important clinical pathogen.\u003c\/p\u003e\u003c\/div\u003e\u003cbr\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublication Date: \u003c\/td\u003e\n\u003ctd\u003e08 May 2026\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublisher: \u003c\/td\u003e\n\u003ctd\u003eSpringer US\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImprint: \u003c\/td\u003e\n\u003ctd\u003eHumana\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eISBN-13: \u003c\/td\u003e\n\u003ctd\u003e9781071653272\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat: \u003c\/td\u003e\n\u003ctd\u003eHardback\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePage Count: \u003c\/td\u003e\n\u003ctd\u003e244\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Springer US","offers":[{"title":"Default Title","offer_id":46544925130892,"sku":"9781071653272","price":197.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071653272.jpg?v=1780593613","url":"https:\/\/lateknightbooks.com\/products\/9781071653272","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}