{"product_id":"9781071653876","title":"Fusobacterium nucleatum Methods and Protocols","description":"\u003ch3\u003eMethods in Molecular Biology\u003c\/h3\u003e\u003ch1\u003eFusobacterium nucleatum\u003c\/h1\u003e\u003ch2\u003eMethods and Protocols\u003c\/h2\u003e\u003ch3\u003eHung Ton-That\u003c\/h3\u003e\u003cdiv\u003e\u003cb\u003eScience \/ Life Sciences \/ Microbiology\u003c\/b\u003e\u003c\/div\u003e\u003cbr\u003e\u003cdiv\u003e\u003cp class=\"MsoNormal\"\u003eThis volume presents a collection of both classic and modern experimental methods designed to highlight the biology, pathogenicity, and host interactions of \u003cem\u003eFusobacterium nucleatum\u003c\/em\u003e. The chapters in this book provide practical guidance spanning microbiology, molecular biology, imaging, genetic engineering, metabolomics, and in vivo modeling. Some of the topics discussed in this volume are methods for visualizing and isolating \u003cem\u003eF. nucleatum\u003c\/em\u003e within its native oral environment; essential cellular processes such as surface protein assembly and peptidoglycan biosynthesis; protocols for asymmetric coculture systems; 3D tumor spheroid models; untargeted metabolite profiling of infected mammalian cells; and several in vivo platforms – from murine models of periodontitis, oral cancer, and CRC to a \u003cem\u003eCaenorhabditis elegans\u003c\/em\u003e infection model. Written in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003cbr\u003eComprehensive and cutting-edge, \u003cem\u003eFusobacterium nucleatum: Methods and Protocols \u003c\/em\u003eis a fundamental resource for both new and experienced researchers looking to investigate \u003cem\u003eF. nucleatum \u003c\/em\u003evirulence mechanisms, colonization strategies, and its increasing contributions to diseases such as CRC, oral squamous cell carcinoma, and periodontitis.\u003c\/p\u003e\u003c\/div\u003e\u003cbr\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublication Date: \u003c\/td\u003e\n\u003ctd\u003e20 July 2026\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePublisher: \u003c\/td\u003e\n\u003ctd\u003eSpringer US\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImprint: \u003c\/td\u003e\n\u003ctd\u003eHumana\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eISBN-13: \u003c\/td\u003e\n\u003ctd\u003e9781071653876\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat: \u003c\/td\u003e\n\u003ctd\u003eHardback\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePage Count: \u003c\/td\u003e\n\u003ctd\u003e216\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Springer US","offers":[{"title":"Default Title","offer_id":47385987285132,"sku":"9781071653876","price":197.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781071653876.jpg?v=1781057434","url":"https:\/\/lateknightbooks.com\/products\/9781071653876","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}