This fifth edition volume expands on the previous editions with updates on the latest advancements in immobilizing enzymes and cells and its importance, and further guides researchers in designing, fabricating, and characterizing heterogeneous biocatalysts, including both immobilized enzymes and cells. The chapters in this book are organized into six parts and illustrate the most innovative protocols to fabricate heterogeneous biocatalysts with better properties than the soluble enzymes under reaction conditions with industrial relevance. This important task requires combining knowledge from material sciences, surface chemistry, protein chemistry, biophysics, molecular biology, biocatalysis, and chemical engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Immobilization of Enzymes and Cells, Fifth Edition is a valuable resource for researchers interested in expanding their knowledge of this important and advancing field.