This second edition provides new and updated chapters on methods for profiling and interpreting genomic alterations in tumors across both research and clinical settings. Chapters detail methods to interrogate DNA variation, RNA expression, and epigenetic changes using both next-generation sequencing and microarray techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Tumor Profiling: Methods and Protocols, Second Edition aims to provide methods to generate and analyze data types to produce a molecular profile of tumors.