This volume details Next-generation probiotics (NGPs) and their development for specific therapeutic uses, unlike traditional probiotics for general supplements.Chapters guide readers through next-generation probiotics, sources of isolation of probiotics, Next-Generation probiotics culture dependent, isolation of the Next-Generation probiotics, culturing techniques (culturomics), in vivo studies to validate the safety and efficacy of NGP,extraction of and quantification of metagenomics DNA, Illumina sequencing platform, whole genome assembly, specific molecular pathways of the bacteria present, and metatranscriptomics analysis of Next-Generation Probiotics from food samples and stool samples. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols and contain notes on how to avoid and solve typical problems.

 Authoritative and cutting-edge, Next Generation Probiotics: Food and Feed Industry provides well-established protocols used by both academics and industrials.