This new volume collects protocols that cover diverse aspects of epidermal cell research and exemplify the exciting progress and improvements in the field and the important areas to build upon for continued development. The book highlights how understanding the development and responsiveness of the epidermis to physiological and pathological cues is crucial for maintaining their function and, ultimately, for maintaining health, and explores methodological advances in the isolation, culturing, and use of epidermal cells to generate in vitro epidermis-like structures. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Epidermal Cells: Methods and Protocols, Fifth Edition serves as a valuable reference for experts as well as novices in the epidermal cell field.