This second edition volume expands on the previous edition with a comprehensive overview of the fundamental aspects of RNA editing, including its detection, biological roles, involvement in human disorders, and interactions with other molecular mechanisms. This volume also explores the most recent technological advances that are paving the way for therapeutic applications and supporting the emerging paradigm of precision medicine. The chapters in this book cover four major parts: RNA editing profiling in non-human organisms; methodological advancement in RNA editing detection; biological roles and functions of RNA editing; and programmable RNA editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, RNA Editing: Methods and Protocols, Second Edition is a practical and valuable resource for all researchers interested in learning more about this interesting and developing field.

Chapter 22 and 23 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.