This third edition volume expands on the previous editions with discussions on the latest advancements used to study all aspects of non-coding RNAs biology, from their isolation and precipitation, through their quantification, accurate detection, functional analysis and high-throughput or even structural analysis. The chapters in this book are dedicated to biochemists, cellular/molecular biologists, or physicists already working in the field of RNA biology, or who want to start studying non-coding RNAs in their projects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Small Non-Coding RNAs: Methods and Protocols, Third Edition is a valuable resource that will help all researchers interested in studying the numerous functions of non-coding RNAs, both on the fundamental and clinical levels.