{"product_id":"9781493915675","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eHughes, Michael R.; McNagny, Kelly M.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eMast Cells: Methods and Protocols, Second Edition \u003c\/i\u003eexpands upon the previous edition with current, detailed reviews that cover topics of interest to mast cell neophytes and \u003ci\u003ecognoscenti\u003c\/i\u003e alike.  With new chapters on methods for the enumeration of tissue mast cells and isolation of mature mast cells and mast cell progenitors from mammalian tissues, \u003ci\u003eDanio rerio \u003c\/i\u003e(zebrafish) model in the study of mast cell development and function, the functions of mast cells in human health and disease and methods for the isolation, methods for the investigation of the molecular mechanisms of mast cell activation and their effector functions, assays for the detection and analysis of mast cell secretory and cell surface phenotype and, mast cell activation state, and experimental mouse models of disease that have been deemed useful for the assessment of mast cell functions in the regulation of innate and adaptive immune response in cancer, tissue fibrosis, auto-inflammation and allergicdisease. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003eAuthoritative and cutting-edge, \u003ci\u003eMast Cells: Methods and Protocols, Second Edition\u003c\/i\u003e provides a sampler of useful methods and techniques in the further study of mast cell biology.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2014-11-13\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493915675\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-1568-2\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 540\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542813462668,"sku":"9781493915675","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493915675.jpg?v=1772844049","url":"https:\/\/lateknightbooks.com\/products\/9781493915675","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}