{"product_id":"9781493936236","title":"Methods in Molecular Biology: Production, Introduction Into Cells, and Physiological Consequences","description":"\u003ch1\u003eMethods in Molecular Biology: Production, Introduction Into Cells, and Physiological Consequences\u003c\/h1\u003e \u003ch2\u003eRhoads, Robert E.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume\npresents detailed laboratory protocols for \u003ci\u003ein\nvitro\u003c\/i\u003e synthesis of mRNA with favorable properties, its introduction into\ncells by a variety of techniques, and the measurement of physiological and\nclinical consequences such as protein replacement and cancer immunotherapy. Synthetic\ntechniques are described for structural features in mRNA that provide investigational\ntools such as fluorescence emission, click chemistry, photo-chemical\ncrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced\nactivation of the innate immune response. Protocols are described for clinical\napplications such as large-scale transfection of dendritic cells, production of\nGMP-grade mRNA, redirecting T cell specificity, and use of molecular\nadjuvants for RNA vaccines. Written\nin the highly successful \u003ci\u003eMethods in\nMolecular Biology\u003c\/i\u003e series format, chapters include introductions to their\nrespective topics, lists of the necessarymaterials and reagents, step-by-step\nlaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\u003ci\u003e\u003cbr\u003eSynthetic\nmRNA: Production, Introduction into Cells, and Physiological Consequences \u003c\/i\u003eis a valuable and cutting-edge resource\nfor both laboratory investigators and clinicians interested in this powerful\nand rapidly evolving technology. \u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-05-29\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493936236\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-3625-0\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 320\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542822932620,"sku":"9781493936236","price":179.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493936236.jpg?v=1772844463","url":"https:\/\/lateknightbooks.com\/products\/9781493936236","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}