{"product_id":"9781493958252","title":"Methods in Molecular Biology: Concepts, Methods, and Applications","description":"\u003ch1\u003eMethods in Molecular Biology: Concepts, Methods, and Applications\u003c\/h1\u003e \u003ch2\u003evan Rij, Ronald P.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eViruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi.\u003ci\u003e Antiviral RNAi: Concepts, Methods, and Applications \u003c\/i\u003eprovides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003eAuthoritative and accessible, \u003ci\u003eAntiviral RNAi: Concepts, Methods, and Applications\u003c\/i\u003e serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-23\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493958252\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-61779-037-9\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 425\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542835679372,"sku":"9781493958252","price":107.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493958252.jpg?v=1772845220","url":"https:\/\/lateknightbooks.com\/products\/9781493958252","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}