{"product_id":"9781493959976","title":"Methods in Molecular Biology","description":"\u003ch1\u003eMethods in Molecular Biology\u003c\/h1\u003e \u003ch2\u003eEichinger, Ludwig; Rivero, Francisco\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eDictyostelium discoideum\u003c\/i\u003e is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. \u003ci\u003eDictyostelium\u003c\/i\u003e is also increasingly used for the investigation of human disease genes and the crosstalk between host and pathogen. The availability of the genome sequence at a mouse click together with a whole range of supporting information and resources along with a powerful armoury of molecular genetics techniques have considerably enhanced the experimental attractiveness of \u003ci\u003eD. discoideum\u003c\/i\u003e in recent years.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eThe second edition of \u003ci\u003eDictyostelium discoideum Protocols\u003c\/i\u003e incorporates the most recent developments in a number of fields. The book is divided into four parts. The first part provides an introduction to the amoebozoa and community resources. The second part presents large-scale analysis methods made possible by the completion of the \u003ci\u003eDictyostelium\u003c\/i\u003e genome sequence. The third part is dedicated to molecular genetics techniques, cell biological, biochemical and biophysical methods. The chapters in the fourth part describe the use of \u003ci\u003eDictyostelium\u003c\/i\u003e as a model system to study vesicle formation, trafficking and infection by bacterial pathogens. \u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eWritten in the highly successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e Authoritative and practical, \u003ci\u003eDictyostelium discoideum Protocols, Second Edition \u003c\/i\u003eseeks to aid scientists in the application of innovating techniques to study a range of fundamental biological processes in this attractive model organism.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-23\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493959976\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-62703-302-2\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 479\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542836990092,"sku":"9781493959976","price":107.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493959976.jpg?v=1772845313","url":"https:\/\/lateknightbooks.com\/products\/9781493959976","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}