{"product_id":"9781493961757","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eSnyder, Richard O.; Moullier, Philippe\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eToday, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of  gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In \u003ci\u003eAdeno-Associated Virus: Methods and Protocols\u003c\/i\u003e, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eAuthoritative and accessible, \u003ci\u003eAdeno-Associated Virus: Methods and Protocols\u003c\/i\u003e provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-23\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493961757\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-61779-370-7\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 462\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542838071436,"sku":"9781493961757","price":161.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493961757.jpg?v=1772845392","url":"https:\/\/lateknightbooks.com\/products\/9781493961757","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}