{"product_id":"9781493962877","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eSamuelson, James C.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eWhether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. \u003ci\u003eEnzyme Engineering: Methods and Protocols\u003c\/i\u003e provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eAuthoritative and easily accessible\u003ci\u003e Enzyme Engineering: Methods and Protocols\u003c\/i\u003e will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-23\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493962877\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-62703-293-3\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 252\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542832369804,"sku":"9781493962877","price":107.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493962877.jpg?v=1772845038","url":"https:\/\/lateknightbooks.com\/products\/9781493962877","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}