{"product_id":"9781493963225","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eOssipow, Vincent; Fischer, Nicolas\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003e\u003ci\u003eMonoclonal Antibodies: Methods and Protocols\u003c\/i\u003e, \u003ci\u003eSecond Edition\u003c\/i\u003e expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003eAuthoritative and practical, \u003ci\u003eMonoclonal Antibodies: Methods and Protocols, Second Edition\u003c\/i\u003e provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-08-23\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493963225\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-62703-992-5\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 575\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":45988191371404,"sku":"9781493963225","price":215.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493963225.jpg?v=1775753598","url":"https:\/\/lateknightbooks.com\/products\/9781493963225","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}