{"product_id":"9781493965229","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eRani, Sweta\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eThis volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of \u003ci\u003eMicroRNA Profiling: Methods and Protocols \u003c\/i\u003ediscuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e\u003cbr\u003eComprehensive and cutting-edge, \u003ci\u003eMicroRNA Profiling: Methods and Protocols\u003c\/i\u003e is a valuable resource for anyone interested in the field of Micro RNAs.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2016-11-09\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493965229\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-6524-3\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 246\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542872051852,"sku":"9781493965229","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493965229.jpg?v=1772846092","url":"https:\/\/lateknightbooks.com\/products\/9781493965229","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}