{"product_id":"9781493975211","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eBoon, Camiel J.F.; Wijnholds, Jan\u003c\/h2\u003e \u003cp\u003eThis detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. \u003c\/p\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and practical, \u003ci\u003eRetinal Gene Therapy: Methods and Protocols\u003c\/i\u003e provides a wide range of readers from students to research experts with vital information on ocular gene therapy vector technology, \u003ci\u003ein vitro\u003c\/i\u003e and \u003ci\u003ein vivo\u003c\/i\u003e biological assays, and clinical protocols, to promote further studies for the benefit of children and adults with inherited retinal disease.\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2017-11-29\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493975211\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-7522-8\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 380\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542897840268,"sku":"9781493975211","price":161.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493975211.jpg?v=1772846492","url":"https:\/\/lateknightbooks.com\/products\/9781493975211","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}