Methods in Molecular Biology: Methods and Protocols

Hust, Michael; Lim, Theam Soon

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.

Details

Published by: Humana

Publication Date: 2018-08-30

Format: Paperback

ISBN-13: 9781493984886

DOI: 10.1007/978-1-4939-7447-4

Dimensions: 254cm x178cm

Pages: 562