Methods in Molecular Biology: Design and Delivery

Castle, Michael J.

This volume provides a complete and timely guide to the use of adeno-associated virus (AAV)  vectors for genetic manipulation of mammalian tissues. Beginning with methods for the design and characterization of AAV vectors, the book continues with protocols for AAV delivery to various components of the central nervous system, to a number of sensory systems, and to a broad range of other tissues. Novel techniques such as ultrasound-targeted delivery to the brain, subpial delivery to the spinal cord, and subILM delivery to the retina are accompanied by chapters that provide an overview and comparison of current methods for AAV delivery to tissues such as brain, heart, liver, and lung. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 

Details

Published by: Humana

Publication Date: 2019-02-20

Format: Hardcover

ISBN-13: 9781493991389

DOI: 10.1007/978-1-4939-9139-6

Dimensions: 254cm x178cm

Pages: 426