Methods in Molecular Biology: Methods and Protocols

Sarwal, Minnie M.; Sigdel, Tara K.

This book presents a compilation of methods that detail improved protein and peptide sample preparation and identification. Chapters guide readers through methods for depletion of myofibril-associated proteins, peptide sample preparation in urinary proteomics, purification of targeted proteins from native tissues, fractionation strategies for protein analysis, and GeLC-MS as a sample preparation method for sample preparation for proteomics using minimal amount of tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Tissue Proteomics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Details

Published by: Humana

Publication Date: 2019-12-10

Format: Paperback

ISBN-13: 9781493993048

DOI: 10.1007/978-1-4939-7854-0

Dimensions: 254cm x178cm

Pages: 320