{"product_id":"9781493993109","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eOñate-Sánchez, Luis\u003c\/h2\u003e \u003cp\u003eThis detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions.  The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and\/or quantitatively) those observed in yeast. Written for the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series, chapters include thekind of detail and key implementation advice that leads to excellent results in the lab. \u003c\/p\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e\u003cdiv\u003eAuthoritative and practical, \u003ci\u003eTwo-Hybrid Systems: Methods and Protocols\u003c\/i\u003e serves as an ideal guide to the uses of this exceedingly valuable technique.\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2019-07-25\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493993109\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-7871-7\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 355\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46542919499916,"sku":"9781493993109","price":161.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493993109.jpg?v=1772847729","url":"https:\/\/lateknightbooks.com\/products\/9781493993109","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}