{"product_id":"9781493998166","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eDiefenbach, Russell J.; Fraefel, Cornel\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cdiv\u003eThis second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR\/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful \u003ci\u003eMethods in Molecular Biology\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.\u003cbr\u003e\n\u003c\/div\u003e\u003cdiv\u003e \u003c\/div\u003e\u003cdiv\u003eCutting-edge and comprehensive, \u003ci\u003eHerpes Simplex Virus: Methods and Protocols, Second Edition\u003c\/i\u003e is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and\/or cellular-based approaches to study HSV. \u003c\/div\u003e\u003cdiv\u003e\u003cbr\u003e\u003c\/div\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2020-10-16\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781493998166\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-4939-9814-2\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 457\u003c\/p\u003e ","brand":"Springer New York","offers":[{"title":"Default Title","offer_id":46543044214924,"sku":"9781493998166","price":116.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781493998166.jpg?v=1772848307","url":"https:\/\/lateknightbooks.com\/products\/9781493998166","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}