Springer Protocols Handbooks

Walker, John M.; Rapley, Ralph

John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.

Details

Published by: Humana

Publication Date: 2005-03-18

Format: Paperback

ISBN-13: 9781588293343

DOI: 10.1385/1592598706

Dimensions: 254cm x178cm

Pages: 644