{"product_id":"9781603271745","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eBarker, Christopher J.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eInositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In \u003cem\u003eInositol Phosphates and Lipids: Methods and Protocols\u003c\/em\u003e, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\n\u003cp\u003e\u003c\/p\u003e\n\u003cp\u003eAuthoritative and cutting-edge, \u003cem\u003eInositol Phosphates and Lipids: Methods and Protocols\u003c\/em\u003e compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2010-05-27\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781603271745\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-60327-175-2\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 230\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542603550860,"sku":"9781603271745","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781603271745.jpg?v=1772841753","url":"https:\/\/lateknightbooks.com\/products\/9781603271745","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}