{"product_id":"9781607616283","title":"Methods in Molecular Biology: Second Edition","description":"\u003ch1\u003eMethods in Molecular Biology: Second Edition\u003c\/h1\u003e \u003ch2\u003eKing, Nicola\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eOnce a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In \u003cem\u003eRT-PCR Protocols, Second Edition\u003c\/em\u003e, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful \u003cem\u003eMethods in Molecular Biology\u003c\/em\u003e™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\n\u003cp\u003eUser friendly and up-to-date, \u003cem\u003eRT-PCR Protocols, Second Edition\u003c\/em\u003e acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2010-03-22\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781607616283\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-60761-629-0\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 341\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542558265484,"sku":"9781607616283","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781607616283.jpg?v=1772841625","url":"https:\/\/lateknightbooks.com\/products\/9781607616283","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}