{"product_id":"9781617374418","title":"Methods in Molecular Biology: Methods and Applications","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Applications\u003c\/h1\u003e \u003ch2\u003eHerdewijn, Piet\u003c\/h2\u003e \u003cp\u003eA collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding  of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2010-11-10\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781617374418\u003c\/p\u003e \u003cp\u003eDOI: 10.1385\/1592598234\u003c\/p\u003e \u003cp\u003eDimensions: 229cm x152cm\u003c\/p\u003e \u003cp\u003ePages: 436\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542639366284,"sku":"9781617374418","price":179.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781617374418.jpg?v=1772842444","url":"https:\/\/lateknightbooks.com\/products\/9781617374418","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}