{"product_id":"9781617375293","title":"Methods in Molecular Biology","description":"\u003ch1\u003eMethods in Molecular Biology\u003c\/h1\u003e \u003ch2\u003eOsterman, Andrei L.; Gerdes, Svetlana Y.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eMicrobial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular Biology™ series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2010-11-19\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9781617375293\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-59745-321-9\u003c\/p\u003e \u003cp\u003eDimensions: 235cm x155cm\u003c\/p\u003e \u003cp\u003ePages: 486\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542611218572,"sku":"9781617375293","price":98.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781617375293.jpg?v=1772841815","url":"https:\/\/lateknightbooks.com\/products\/9781617375293","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}