Methods in Molecular Biology: Reviews and Protocols

Minteer, Shelley D.

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Details

Published by: Humana

Publication Date: 2010-12-09

Format: Paperback

ISBN-13: 9781617376139

DOI: 10.1385/1592599974

Dimensions: 229cm x152cm

Pages: 248