{"product_id":"9781617791963","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eWilliams, James A.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eClassical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In \u003ci\u003eStrain Engineering: Methods and Protocols\u003c\/i\u003e, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, \u003ci\u003eE. coli\u003c\/i\u003e-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast \u003ci\u003eSaccharomyces cerevisiae\u003c\/i\u003e as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003eAuthoritative and accessible, \u003ci\u003eStrain Engineering: Methods and Protocols\u003c\/i\u003e serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2011-07-15\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781617791963\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-61779-197-0\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 480\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542737375372,"sku":"9781617791963","price":152.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781617791963.jpg?v=1772842844","url":"https:\/\/lateknightbooks.com\/products\/9781617791963","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}