{"product_id":"9781617798818","title":"Methods in Molecular Biology: Methods and Protocols","description":"\u003ch1\u003eMethods in Molecular Biology: Methods and Protocols\u003c\/h1\u003e \u003ch2\u003eWatson, John M.; Wang, Ming-Bo\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eStudies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. In \u003ci\u003eAntiviral Resistance in Plants: Methods and Protocols\u003c\/i\u003e, expert researchers in the field detail many of the methods which are now commonly used to study the phenomenon of RNA silencing in relation to viral infections of plants.  These include methods and techniques for the isolation and quantitative\/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes.  Written in the highly successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eAuthoritative and practical, \u003ci\u003eAntiviral Resistance in Plants: Methods and Protocols\u003c\/i\u003e seeks to aid scientists in the further study of this crucially important botanical trait. \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2012-06-12\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781617798818\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-61779-882-5\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 380\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542790754444,"sku":"9781617798818","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781617798818.jpg?v=1772843188","url":"https:\/\/lateknightbooks.com\/products\/9781617798818","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}