{"product_id":"9781617799082","title":"Methods in Molecular Biology","description":"\u003ch1\u003eMethods in Molecular Biology\u003c\/h1\u003e \u003ch2\u003eDavenport, Anthony P.\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eA broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand  to undergo a conformational change, leading to a physiological response or change in cell function.  A ligand can be an endogenous neurotransmitter, hormone, paracrine\/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of  \u003ci\u003eReceptor Binding Techniques \u003c\/i\u003eexpands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of  immunocytochemistry  for the cellular localization of receptor protein and hybridization to detect receptor mRNA.  Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and  green fluorescent protein to study  trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel ‘biased agonists’. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET). \u003c\/p\u003e\u003cp\u003eWritten in the highly successful \u003ci\u003eMethods in Molecular Biology™\u003c\/i\u003e series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,\u003ci\u003e Receptor Binding Techniques, Third Edition,\u003c\/i\u003e aids scientists in continuing to study receptor binding. \u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Humana\u003c\/p\u003e \u003cp\u003ePublication Date: 2012-06-08\u003c\/p\u003e \u003cp\u003eFormat: Hardcover\u003c\/p\u003e \u003cp\u003eISBN-13: 9781617799082\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-1-61779-909-9\u003c\/p\u003e \u003cp\u003eDimensions: 254cm x178cm\u003c\/p\u003e \u003cp\u003ePages: 309\u003c\/p\u003e ","brand":"Humana Press","offers":[{"title":"Default Title","offer_id":46542790983820,"sku":"9781617799082","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9781617799082.jpg?v=1772843194","url":"https:\/\/lateknightbooks.com\/products\/9781617799082","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}