This book gathers peer-reviewed contributions to the 13th International Conference on E-Health and Bioengineering (EHB 2025), held in hybrid format on November 13–14, 2025, in/from Iași, Romania. They report on current advances in healthcare assessment, biomedical signal processing and assistive devices. They also cover applications of artificial intelligence in the biomedical field and of bioengineering methods in aerospace. Second volume of a three-volume set this book offers extensive and timely information to researchers and professionals in bioengineering, health informatics, and related interdisciplinary fields.