This book provides practical insights into the latest developments and innovations in the field of learning factories, with a particular focus on next-generation approaches in engineering education and manufacturing systems. The book compiles selected contributions from the 16th Conference on Learning Factories (CLF 2026), held from May 5 to 7, 2026, at the Free University of Bozen-Bolzano. The proceedings cover five fundamental areas: (1) enabling technologies, (2) engineering education, (3) learning factory operations, (4) learning factory concepts and infrastructure, and (5) next-generation agile learning factories. This book is designed for a diverse audience. Researchers will find approaches to current research topics related to learning factories. Practitioners will gain insights from real-world applications and innovative learning factory solutions. Doctoral students and lecturers will gain insights into modern teaching methodologies and the integration of experiential learning environments into engineering education.