This Book constitutes the revised selected papers of the 20th International Meeting on Computational Intelligence Methods for Bioinformatics and Biostatistics, CIBB 2025, held in Milan, Italy, during September 10–12, 2025. 

The 27 full papers presented were carefully reviewed and selected from 120 submissions. The conference focuses on emerging trends and future directions at the intersection of computational and life sciences, with particular interest in challenging problems in bioinformatics, biostatistics, and medical informatics. The full papers are organized in two sections: 'Bioinformatics and Network Modelling' and 'Machine Learning for Medical Informatics.