This volume constitutes the proceedings of the Second International Conference on Real Emotions and Affect in Extended Realities, REALITIES 2025, held in Milan, Italy, on November 21, 2025.

The 7 full papers and 3 short papers were carefully reviewed and selected from 29 submissions. These papers were organized in following topical sections: Immersive Technologies and Human Transformation; Fear Learning, Emotion, and Cognitive Regulation; Brain Stimulation and Mental Health; and Artificial Intelligence, Media, and Extended Cognition.