This book constitutes the refereed proceedings of the 16th International Conference on Passive and Active Measurement, PAM 2015, held in New York, NY, USA, in March 2015. The 27 full papers presented were carefully reviewed and selected from 100 submissions. The papers have been organized in the following topical sections: DNS and Routing, Mobile and Cellular, IPv6, Internet-Wide, Web and Peer-to-Peer, Wireless and Embedded, and Software Defined Networking.