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This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.
Published by: Springer
Publication Date: 2012-07-07
Format: Paperback
ISBN-13: 9783642489778
DOI: 10.1007/978-3-642-71965-3
Dimensions: 235cm x155cm
Pages: 169