{"product_id":"9789400795266","title":"MicroRNAs as Tools in Biopharmaceutical Production","description":"\u003ch1\u003eMicroRNAs as Tools in Biopharmaceutical Production\u003c\/h1\u003e \u003ch2\u003eBarron, Niall\u003c\/h2\u003e \u003cp\u003e\u003c\/p\u003e\u003cp\u003eFocused manuscript on the potential use\/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics\/chapters along the following lines: Intro on CHO\/bioprocessing\/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells,  miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient\/stable disregulation, Knockout.\u003c\/p\u003e \u003ch3\u003eDetails\u003c\/h3\u003e \u003cp\u003ePublished by: Springer\u003c\/p\u003e \u003cp\u003ePublication Date: 2014-12-14\u003c\/p\u003e \u003cp\u003eFormat: Paperback\u003c\/p\u003e \u003cp\u003eISBN-13: 9789400795266\u003c\/p\u003e \u003cp\u003eDOI: 10.1007\/978-94-007-5128-6\u003c\/p\u003e \u003cp\u003eDimensions: 235cm x155cm\u003c\/p\u003e \u003cp\u003ePages: 126\u003c\/p\u003e ","brand":"Springer Netherlands","offers":[{"title":"Default Title","offer_id":44542717493388,"sku":"9789400795266","price":98.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0710\/9545\/1788\/files\/9789400795266.jpg?v=1775833450","url":"https:\/\/lateknightbooks.com\/products\/9789400795266","provider":"Late Knight Books and Services, LLC","version":"1.0","type":"link"}