The world population will exceed 9.7 billion by 2050, creating a huge pressure on agricultural systems to produce sufficient, safe, and nutritious food for growing population. Genetic engineering is the main approach that can fulfil the increasing demands of food for the constantly growing world population.

This book covers the latest information on the development of transgenic plants and addresses the essential aspects and troubleshooting strategies in gene cloning, encompassing comprehensive knowledge about molecular biology and biotechnology. It discusses CRISPR-Cas, a powerful, precise, fast, and cost-effective genome-editing tool that is transforming modern agriculture, and how bioinformatics plays a key role in the characterization of the gene and the promoter at every stage of transgenic plant development. Moreover, the text explains advanced expression and regulatory cloning vector series, characteristics, and their host information. To make society and people aware about transgenic plants, the book also covers the global cultivation, production, and consumption of transgenic plants.

Hardeep Singh Tuli is a professor at the Department of Bio-Sciences and Technology, Maharishi Markandeshwar (Deemed to be University), India. Previously, served as a Lecturer at the Department of Applied Sciences, Institute of Science and Technology, Klawad, Yamunanagar (2009-2011), India. His research interests are focused on the isolation, characterization and biochemical evaluation of natural metabolites as anticancer agents. He has served as a reviewer for several international journals, and as a guest editor for the journal Frontiers in Pharmacology. He has more than 10 years of teaching and research experience in pharmacology, mammalian physiology, and natural products. He has published more than 300 papers in peer-reviewed international journals and authored a number of book chapters. He edited three international books in the area of natural products and chemoprevention. In addition, Dr. Tuli is an editorial board member of various national and international scientific research journals. He is Listed in World's Top 2% Scientists, 2021, 2022, 2023, 2024, 2025.

Pooja Sharma is an assistant professor in the Department of Bio-Sciences and Technology, Maharishi Markandeshwar (Deemed to be University), India. Her doctoral degree focused on molecular characterization of APETALA2 gene from Arabidopsis and Brassica. She has been consistently contributing to the field of molecular biology, gene editing, and agricultural sustainability and has published more than 40 articles and 3 books. She has participated in several scientific conferences, workshops, webinars, and academic events. Dr Sharma has been invited as a speaker at various prestigious international as well as national platforms.

Soniya Goyal is an assistant professor in the Department of Biosciences and Technology at Maharishi Markandeshwar (Deemed to be University). Her doctoral research focused on the molecular analysis of multidrug-resistant Escherichia coli, and she has continued her research in antimicrobial resistance and endophytic fungi in healthcare and agriculture. She has more than 7 years of research experience in biosciences research, teaching, and administration. Dr Goyal has published more than 19 articles and several book chapters.